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RECORD NUMBER: 93 OF 134

OLS Field Name OLS Field Data
Main Title Preparation and Characterization of Monoclonal Antibodies to Enteric Adenovirus Types 40 and 41.
Author Herrmann, J. E. ; Perron-Henry, D. M. ; Stobbs-Walro, D. ; Blacklow, N. R. ;
CORP Author Massachusetts Univ. Medical School, Worcester.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/216;
Stock Number PB88-170659
Additional Subjects Viruses ; Antibodies ; Reprints ; Monoclonal antibodies ; Enteric adenoviruses ; Cell lines
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NTIS  PB88-170659 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 9p
Abstract
The authors have prepared monoclonal antibodies to each of the enteric adenoviruses types 40 and 41. Three different hybridoma cell lines were selected which produced antibody found to react by radioimmunoprecipitation with adenovirus (Ad) hexon antigens. One was specific for Ad40, another for Ad41, and a third one reacted with both types. When tested in an enzyme immunoassay against all 41 known human Ad types, the type-specific monoclonal antibody against Ad40 reacted homotypically, as did the monoclonal antibody against Ad41. In addition, these monoclonal antibodies neutralized the homologous enteric Ad type. The monoclonal antibody which reacted with both enteric Ad types also showed lower levels of reactivity with the Group C adenoviruses types 2,5, and 6. The monoclonal antibodies produced will provide a definitive means for rapid identification of specific Ad types, and will be useful in defining the relationship of enteric adenovirsuses to other types.