Record Display for the EPA National Library Catalog

RECORD NUMBER: 30 OF 37

OLS Field Name OLS Field Data
Main Title Poly(1):poly(C)-Enhanced Alveolar and Peritoneal Macrophage Phagocytosis: Quantification by a New Method Utilizing Fluoroescent Beads.
Author Burleson, G. R. ; Fuller, L. B. ; Menache, M. G. ; Graham, J. A. ;
CORP Author Northrop Services, Inc., Research Triangle Park, NC.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-68-02-4032; EPA/600/J-87/092;
Stock Number PB88-125471
Additional Subjects Polystyrene ; Respiration ; Toxicology ; Immunology ; Rats ; Alveoli pulmonis ; Beads ; Reprints ; Phagocytosis ; Macrophages ; Interferon inducers
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB88-125471 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 10p
Abstract
A technique for quantifying nonspecific phagocytosis of alveolar and peritoneal macrophages in the same animal has been developed utilizing fluorescent polystyrene beads. When incorporated into inhalation studies, the technique can be used to determine whether the toxic effect of an inhaled pollutant is local (effect on alveolar macrophages), systemic (effect on peritoneal macrophages), or both local and systemic. The method results in a determination of the percent phagocytic macrophages and of the degree of individual macrophage phagocytic activity based on the number of beads phagocytized per macrophage. Macrophage preparations were incubated with fluorescent beads for 2 hr and cytocentrifuged onto a glass slide. Fluorescent beads present on the slide or cell-associated but not ingested by phagocytosis were removed by immersing the slide containing the macrophago preparation in methylene chloride for 30-60 sec. Fluorescent beads ingested by phagocytosis were then easily quantified with a fluorescence microscope. (Copyright (c) 1987 by the Society for Experimental Biology and Medicine.)