||Microinjection of Chemical Carcinogens into Small Fish Embryos: Exocrine Pancreatic Neoplasm in 'Fundulus grandis' Exposed to N-methyl-N'-nitro-N-Nitrosoguanidine.
Grizzle, J. M. ;
Putnam, M. R. ;
Fournie, J. W. ;
Couch, J. A. ;
||Environmental Research Lab., Gulf Breeze, FL. ;Auburn Univ., AL.
Malignant neoplasms ;
Laboratory animals ;
Fundulus grandis ;
Animal models ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Gulf killifish (Fundulus grandis) embryos were injected with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Retention of carcinogen in the egg immediately after injection was highly variable. Hatching percentages of MNNG-injected and uninjected embryos were similar, but survival of MNNG-injected fish during the first week after hatching was less than half of the survival of controls. One of three MNNG-exposed fish examined after 20 weeks had a pancreatic acinar cell carcinoma, a type of neoplasm seldom found in fish. No neoplasms were found in controls nor in MNNG-exposed fish examined after 34 weeks. The neoplasm was invasive and had a high mitotic index, but no metastases were found. The acinar cell origin of the tumor was confirmed by ultrastructural examination of tissue reprocessed from paraffin into epoxy resin. (Copyright (c) Inter-Research.)