Record Display for the EPA National Library Catalog
RECORD NUMBER: 13 OF 19
|OLS Field Name||OLS Field Data|
|Main Title||Kinetic Study on the Inhibition of Hen Brain Neurotoxic Esterase by Mipafox.|
|Author||Soliman, Salah A. ; Curley, August ;|
|CORP Author||Health Effects Research Lab., Research Triangle Park, NC.|
|Additional Subjects||Inhibitors ; Esterases ; Chickens ; Brain ; Reprints ; Mipafox ; Neurotoxins ; Valerate/nitrophenyl|
A direct method of assaying neurotoxic esterase (NTE) activity, using 4-nitrophenyl valerate, has been described. The technique was used to determine the biomolecular rate (ki), phosphorylation (k2), and affinity (kd) constants for the reaction of hen brain microsomal NTE with mipafox. Results indicate that the new technique for assaying NTE makes detailed kinetic studies of NTE inhibition possible.