Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 8

OLS Field Name OLS Field Data
Main Title Initial-Phase Optimization for Bioremediation of Munition Compound-Contaminated Soils.
Author Funk, S. B. ; Roberts, D. J. ; Crawford, D. L. ; Crawford, R. L. ;
CORP Author Idaho Univ., Moscow. Dept. of Bacteriology and Biochemistry.;Environmental Research Lab., Athens, GA. Office of Research and Development.
Publisher cJul 93
Year Published 1993
Report Number EPA-R-820804; EPA/600/J-94/474;
Stock Number PB95-137360
Additional Subjects Soil pollution ; Explosives ; Degradation ; Remedial action ; Reprints ; TNT ; Triazines ; Cultures(Biology) ; Anaerobic processes ; pH ; Biotechnology ; Contamination ; Nitrogen organic compounds ; Nitro compounds ; Polymerization ; Tetraacocine
Holdings
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NTIS  PB95-137360 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/06/1995
Collation 9p
Abstract
We examined the bioremediation of soils contaminated with the munition compounds 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraacocine by a procedure that produced anaerobic conditions in the soils and promoted biodegradation of nitroaromatic contaminants. This procedure consisted of flooding the soils with 50 mM phosphate buffer, adding starch as a supplemental carbon substrate, and incubating under static conditions. Aerobic heterotrophs, present naturally in the soil or added as an inoculum, quickly removed the oxygen from the static cultures, creating anaerobic conditions. Removal of parent TNT molecules from the soil cultures by the strictly anaerobic microflora occurred within 4 days.