||GC/MS Identification of Mutagens in Aqueous Chlorinated Humic Acid and Drinking Waters Following HPLC (High-Pressure Liquid Chromatography) Fractionation of Strong Acid Extracts.
Coleman, W. E. ;
Munch, J. W. ;
Hodakievic, P. A. ;
Kopfler, F. C. ;
Meier, J. R. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Cincinnati Univ., OH. Dept. of Chemistry.
Potable water ;
Humic acids ;
Water treatment ;
Chemical removal(Water treatment) ;
Water pollution ;
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Recent publications indicate that mutagenic substances are formed when both humic material and source water containing significant humic material are chlorinated. Past studies have shown that the strong acid fraction of ether extracts of chlorinated humic acid (CHA) is very mutagenic. Fractionation using HPLC has been used to separate the acid fraction further into subfractions. Ames test results show all the mutagenic activity in two HPLC fractions. The paper discusses the identification of other substances in these mutagenic HPLC fractions and compares the results of analysis of CHA with 3 drinking water samples. The results indicate that water containing humic material and chlorinated under drinking water treatment conditions contains many of the same types of mutagenic compounds found in CHA solutions.