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RECORD NUMBER: 9 OF 70

OLS Field Name OLS Field Data
Main Title Assessing the Use of Known Mutagens to Calibrate the 'Salmonella typhimurium' Mutagenicity Assay. 2. With Exogenous Activation.
Author Claxton, L. D. ; Houk, V. S. ; Warner, J. R. ; Myers, L. E. ; Hughes, T. J. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Research Triangle Inst., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/265;
Stock Number PB92-113273
Additional Subjects Salmonella typhimurium ; Mutagenicity tests ; Mutagens ; Metabolic activation ; Dose-response relationships ; Tables(Data) ; Data bases ; Rats ; Liver ; Comparison ; Analysis of variance ; Reprints ; Standard reference materials
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB92-113273 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 02/24/1992
Collation 13p
Abstract
In order to determine the usefulness of selected chemicals as potential reference materials for calibrating the Salmonella assay, two laboratories tested a series of Salmonella mutagens that require exogenous activation. When the variance for individual substances within a bioassay is sufficiently low and the rankings of those substances are of acceptable consistency, they can later be evaluated for use as standard control compounds, as audit materials, and as standard reference materials for comparative bioassay efforts. The purpose of the project, therefore, was to evaluate the variability in the mutagenic response of potential reference chemicals that require exogenous metabolic activation in the standard plate-incorporation Salmonella mutagenicity assay, and to develop ranking criteria for mutagenic activity based on these data. Ten indirect-acting mutagens were tested in two laboratories using Salmonella typhimurium TA100 and an Aroclor-induced rat liver S9. Each laboratory conducted four definitive testing rounds. A different batch of S9 was utilized for every two rounds. Of the 10 chemicals tested only 2-anthramine had a mean slope value greater than 1000 revertants/microgram. Three chemicals had slope values between 1000 and 100; and five chemicals had slope values between 100 and 10. (Copyright (c) 1991 Elsevier Science Publishers B.V.)