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RECORD NUMBER: 25 OF 34

OLS Field Name OLS Field Data
Main Title Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'.
Author Chen, C. ; Turi, T. G. ; Sanglard, D. ; Loper, J. C. ;
CORP Author Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1987
Year Published 1987
Report Number EPA-CR-810605 ;EPA-CR-813366; EPA/600/J-93/303;
Stock Number PB93-229250
Additional Subjects Cytochrome P-450 ; Saccharomyces cerevisiae ; Fungal genes ; Gene expression ; Escherichia coli ; Fungal DNA ; Heme ; Drug resistance ; Ketoconazole ; Molecular sequence data ; Reprints ; Candida tropicalis
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB93-229250 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/22/1993
Collation 9p
Abstract
The gene for cytochrome P450 lanosterol 14alpha-demethylase (14DM) from the yeast Candida tropicalis has been isolated. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by (a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and (b) based upon tests of its expression in strains of Saccharomyces cerevisiae. (Copyright (c) 1987 Academic Press, Inc.)