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RECORD NUMBER: 23 OF 38

OLS Field Name OLS Field Data
Main Title Effect of Pentachlorophenol on the Activation of 2,6-Dinitrotoluene to Genotoxic Urinary Metabolites in CD-1 Mice: A Comparison of GI Enzyme Activities and Urine Mutagenicity.
Author George, S. E. ; Chadwick, R. W. ; Creason, J. P. ; Kohan, M. J. ; Dekker, J. P. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/075;
Stock Number PB92-150895
Additional Subjects Mutagens ; Metabolic activation ; Salmonella typhimurium ; Mutagenicity tests ; Urine ; Bioassay ; Intestines ; Enzymes ; Organ weight ; Multivariate analysis ; Reprints ; Pentachlorophenol ; Dinitrotoluenes
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB92-150895 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/28/1992
Collation 12p
Abstract
2,6-Dinitrotoluene (2,6-DNT) and pentachlorophenol (PCP) are used for industrial purposes and are found in the environment as hazardous contaminants. Because concurrent exposure to both compounds can occur, it is of interest to determine if organochlorine compounds potentiate the effect of nitroaromatic chemicals. CD-1 mice were treated with PCP (42.8 mg/kg) for 4 weeks. On weeks 1,2, and 4 after the initial PCP dose, mice were treated p.o. with 2,6-DNT (75 mg/kg) and 24 hr urines were collected. After concentration, the urines were tested for their mutagenic activity in Salmonella typhimurium strain TA98 without metabolic activation in a microsuspension bioassay. A significant increase (P < .05) in mutagenicity was observed in urines from mice treated with 2,6-DNT alone and in combination with PCP. By week 4, mice that received both 2,6-DNT and PCP excreted urine that was more mutagenic than that from animals which received only 2,6-DNT. At weeks 2 and 4, mice were sacrificed and intestinal enzyme activities (nitroreductase, azo reductase, Beta-glucuronidase, dechlorinase, and dehydrochlorinase) were quantitated. (Copyright (c) 1991 Wiley-Liss, Inc.)