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RECORD NUMBER: 17 OF 38

OLS Field Name OLS Field Data
Main Title Comparison of In vivo and In vitro Methods for Assessing the Effects of Repeated Dosing with Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System (Journal Version).
Author Chadwick, R. W. ; Copeland, M. F. ; Carlson, G. P. ; Trela, B. A. ; Most, B. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology. ;Northrop Services, Inc., Research Triangle Park, NC.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/264;
Stock Number PB89-143705
Additional Subjects Transferases ; Bioassay ; Carbon tetrachloride ; Toxicology ; Hazardous materials ; In vivo analysis ; In vitro analysis ; Rats ; Chlorobenzenes ; Liver ; Experimental design ; Metabolism ; Reprints ; Enzyme repression ; Enzyme tests ; Glutathione transferases ; Comparative evaluations ; Lindane ; Toxic substances ; UDP glucaronyltransferase ; Sulfurtransferases ; Benzene hexachloride ; Dose-response relationships
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Status
NTIS  PB89-143705 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/08/1989
Collation 15p
Abstract
The effect of a single i.p. injection of 0, 20, 200, and 1000 microliters/kg carbon tetrachloride on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay, employing lindane (gamma-hexachlorocyclohexane), and by a battery of in vitro enzyme assays. The data in the study indicated that carbon tetrachloride had a biphasic influence on the phase I reactions with the lowest dose inducing a significant increase in enzyme activity while the highest dose produced significant inhibition. Significant CCl4-induced reductions in glucaronyltransferase and sulfotransferase activities were also observed while the effect on glutathione-S-transferase was ambiguous. The in vivo and in vitro assays showed good agreement. (Copyright (c) 1988 Elsevier Science Publishers B.V.)