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RECORD NUMBER: 15 OF 38

OLS Field Name OLS Field Data
Main Title Comparison of In vivo and In vitro Methods for Assessing the Effects of Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System.
Author Chadwick, R. W. ; Copeland, M. F. ; Carlson, G. P. ; Trela, B. A. ; Most, B. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/545;
Stock Number PB91-109264
Additional Subjects Toxicology ; Carbon tetrachloride ; Liver ; Chlorobenzenes ; Enzymes ; In vitro analysis ; In vivo analysis ; Metabolism ; Insecticides ; Solvents ; Reprints ; UDP glucuronosyltransferase ; Sulfotransferases ; Glutathione transferases ; Cytochrome P-450
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Status
NTIS  PB91-109264 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/04/1991
Collation 10p
Abstract
The effect of a single i.p. injection of 0, 20, 200, and 1000 microliters/kg carbon tetrachloride on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay, employing lindane (gamma-hexachlorocyclohexane), and by a battery of in vitro enzyme assays. The data in the study indicated that carbon tetrachloride had a biphasic influence on the phase I reactions with the lowest dose inducing a significant increase in enzyme activity while the highest dose produced significant inhibition. Significant CCl4-induced reductions in glucuronyltransferase and sulfotransferase activities were also observed while the effect on glutathione-S-transferase was ambiguous. The in vivo and in vitro assays showed good agreement.