||Comparison of In vivo and In vitro Methods for Assessing the Effects of Bromobenzene on the Hepatic-Metabolizing Enzyme System.
Chadwick, R. W. ;
Copeland, M. F. ;
Carlson, G. P. ;
Trela, B. A. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. School of Pharmacy and Pharmacal Sciences.
Bromo organic compounds ;
In vitro analysis ;
In vivo analysis ;
Benzene hexachloride ;
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The effect of daily i.p. injections of 0, 0.05, 0.5 and 5.0 mmol/kg bromobenzene for 1 week on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay employing lindane ( -hexachlorocyclohexane) and by a battery of in vitro enzyme assays. The data in the study indicated that repeated pretreatment with bromobenzene for 1 week stimulated a dose-related increase in phase I reactions while inducing phase II reactions at the high dose (5 mmol/kg bromobenzene). The in vivo and in vitro assays showed good agreement. (Copyright (c) Toxicology Letters, Vol. 39, 1987.)