Record Display for the EPA National Library Catalog

RECORD NUMBER: 2 OF 15

OLS Field Name OLS Field Data
Main Title Differential Recovery of 'tk' and 'hgprt' Induced Mutants in Mammalian Cells.
Author Moore, M. M. ; Brock, K. H. ; DeMarini, D. M. ; Doerr, C. L. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/D-87/226;
Stock Number PB87-212643
Additional Subjects Mutations ; Animal cells ; Genetics ; Mice ; Cells(Biology) ; Culture media ; Mutagen screening ;
Holdings
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Status
NTIS  PB87-212643 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 28p
Abstract
Human genetic disease is known to result from both point mutations and chromosomal aberrations. It is therefore critical that short-term in vitro mammalian tests be evaluated as to their capabilities for detecting both types of lesions. Research to date indicates that L5178Y/TK plus or minus -3.7.2C mouse lymphoma cells permit the measurement of forward mutation at the heterozygous tk locus. Extensive studies indicate that these TK-deficient mutants result from both single-gene mutations (small-colony mutants) as well as mutations involving the expression of multiple loci (large-colony mutants). Comparative mutagenicity studies presented at this Banbury conference reveal a quantitative difference in the ability of the tk and hgprt loci to permit recovery of mutations affecting the expression of multiple loci. The observed HGPRT mutant frequency underestimates the genotoxicity of compounds acting as clastogens.