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RECORD NUMBER: 1 OF 15

OLS Field Name OLS Field Data
Main Title Development of an Intact Hepatocyte Activation System for Routine Use with the Mouse Lymphoma Assay.
Author Brock, K. H. ; Moore, M. M. ; Oglesby, L. A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC.
Year Published 1987
Report Number EPA/600/J-87/194;
Stock Number PB88-165998
Additional Subjects Hepatocytes ; Lymphoma ; In viro analysis ; Mice ; Rats ; Cell cultures ; Reprints ;
Holdings
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Status
NTIS  PB88-165998 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 13p
Abstract
The authors have developed a method for cocultivating primary rat hepatocytes with L5178Y/TK+/- 3.7.2C mouse lymphoma cells. The system should provide a means to simulate more closely in vivo metabolism compared to metabolism by liver homogenates, while still being useful for routine screening. Hepatocytes were isolated from 200-250 g adult male Sprague-Dawley rats. Rapid attachment of the hepatocytes (2 h) was obtained by using fibronectin-coated 25-cm2 tissue culture flasks. Several factors proved important in obtaining optimal cocultivated mouse lymphoma cell growth. These included maintaining the cocultivated cultures on a rocker platform instead of stationary and either (1) reducing the cocultivation time from 16 to 4 h or (2) utilizing less than 1 x 10 to the 6th power hepatocytes per flask for 16-h exposures. CP, DMN, DMBA, and B(a)P were used to demonstrate the capability of the system to detect mutagens that require metabolic activation.