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RECORD NUMBER: 33 OF 33

OLS Field Name OLS Field Data
Main Title V(D)J Recombinase-Mediated Deletion of the 'hprt' Gene in T-Lymphocytes from Adult Humans.
Author Fuscoe, J. C. ; Zimmerman, L. J. ; Harrington-Brock, K. ; Burnette, L. ; Moore, M. M. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Vermont Univ., Burlington. Genetics Lab.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/422;
Stock Number PB93-141216
Additional Subjects Hypoxanthine phosphoribosyltransferase ; T-lymphocyte gene rearrangement ; Mutagenesis ; Chromosome deletion ; Base sequence ; DNA mutational analysis ; Mutations ; Gene expression ; Exons ; Lymphocytes ; Polymerase chain reaction ; Southern blotting ; Adults ; Clone cells ; Newborns ; Reprints ; V(D)J recombinase
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-141216 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/12/1993
Collation 10p
Abstract
The hprt T-cell cloning assay allows the detection of mutations occurring in vivo in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene of T-lymphocytes. In the report, we examined a collection of 314 hprt-deficient clones derived from adult humans for evidence that the mutations were caused by the illegitimate activity of V(D)J recombinase by analyzing exons 2+3 deletion mutations. DNA sequence analysis of deletion breakpoint junctions showed that eight of the mutations were the result of V(D)J recombinase activity. The frequency of the recombinase-mediated mutations was similar in the adults and newborns (2-4 x 10 to the power of -7). Unregulated expression of V(D)J recombinase activity may be an important mechanism for genomic rearrangements in the genesis of cancer.