Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 5

OLS Field Name OLS Field Data
Main Title Formation of DNA Adducts in 1OT1/2 Mouse Embryo Fibroblasts Incubated with Benzo(a)pyrene or Dihydrodiol Oxide Derivatives.
Author Brown, Halina Szejnwald ; Jeffrey, Alan M. ; Weinstein, I. Bernard ;
CORP Author Columbia Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD.
Year Published 1979
Report Number EPA-R-805482 ;PHS-CA-13696; EPA-600/J-79-148;
Stock Number PB83-145292
Additional Subjects Deoxyribonucleic acid ; Aromatic polycyclic hydrocarbons ; Binding ; Mice ; Embryos ; Oxygen organic compounds ; Stereochemistry ; Reprints ; Dihydrodiol oxide ; Metabolites ; Cell lines ; Benzopyrenes ; Fibroblasts
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB83-145292 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 7p
Abstract
Binding of benzo(a)pyrene (BP) metabolites to DNA was studied in the mouse embryo fibroblast 10T one half cell line. The cells metabolized BP to its diastereoisomeric 7,8-dihydrodiol-9,10-oxides, (+)-7B, 8a-dihydroxy-9B, 10B-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, which formed covalent adducts with cellular DNA. Detailed analysis by high-pressure liquid chromatography indicated that a deoxyguanosine adduct, N2-(10S-(7R, 8S, 9R-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yldeoxyguaninosine, was the predominant DNA adduct formed in cells exposed to BP. This adduct is also the major one found in human tissues exposed to BP.