Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Morphological and Cultural Comparison of Microorganisms in Surface Soil and Subsurface Sediments at a Pristine Study Site in Oklahoma (Journal Version).
Author Bone, T. L. ; Balkwill, D. L. ;
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/198;
Stock Number PB89-138820
Additional Subjects Microorganisms ; Sediments ; Ground water ; Soil analysis ; Cell morphology ; Sampling ; Sites ; Oklahoma ; Aquifers. ; Reprints ; Microfloras ; Water pollution sampling ; Land pollution
Library Call Number Additional Info Location Last
NTIS  PB89-138820 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/08/1989
Collation 18p
Surface-soil and subsurface microfloras at the site of a shallow aquifer in Oklahoma were examined and compared with respect to (1) total and viable cell numbers, (2) colony and cell types that grew on various plating media, (3) cell morphologies seen in flotation films stripped from sample particles, and (4) cellular ultrastructure. Appreciable numbers of microbial cells were present in the subsurface, but the subsurface microflora was considerably less populous than that of the surface soil. The subsurface microflora (especially that of the saturated zone) also appeared to be much less diverse, containing fewer microbial types that would grow on enumeration plates (on nutrient-rich media, 3-4 colony types versus 19-22 for the surface soil) and fewer cell types that could be distinguished by direct microscopy (3-4 types versus 17 for the surface soil). The specific types of microorganisms that were numerically predominant in the aquifer sediments were entirely different from those that were predominant in the surface soil. Moreover, the predominant types varied from one depth to another within the saturated zone. The potential metabolic capability of the subsurface microflora, as indicated by its readiness to grow rapidly on nutrient-rich media, also varied with depth. (Copyright (c) Springer-Verlag New York Inc. 1988.)