Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title UAF Radiorespirometric Protocol for Assessing Hydrocarbon Mineralization Potential in Environmental Samples.
Author Brown, E. J. ; Resnick, S. M. ; Rebstock, C. ; Luong, H. V. ; Lindstrom, J. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Alaska Univ., Fairbanks. Inst. of Northern Engineering. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/389;
Stock Number PB93-121218
Additional Subjects Land pollution control ; Oil pollution ; Water pollution control ; Biodeterioration ; Remedial action ; Hydrocarbons ; Oil spills ; Crude oil ; Microorganisms ; Sediments ; Mineralization ; Beaches ; Biomass ; Nitrogen ; Phosphorus ; Carbon dioxide ; Isotopic labeling ; Performance evaluation ; Reprints ; Exxon Valdez ; Radiorespirometry ; Cleanup operations
Library Call Number Additional Info Location Last
NTIS  PB93-121218 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/08/1993
Collation 9p
Following the EXXON Valdez oil spill, a radiorespirometric protocol was developed at the University of Alaska Fairbanks (UAF) to assess the potential for microorganisms in coastal waters and sediments to degrade hydrocarbons. The use of bioremediation to assist in oil spill cleanup operations required microbial bioassays to establish that addition of nitrogen and phosphorus would enhance biodegradation. A technique assessing 1-14C-n-hexadecane mineralization in seawater or nutrient rich sediment suspensions was used for both of these measurements. Hydrocarbon-degradation potentials were determined by measuring mineralization associated with sediment microorganisms in sediment suspended in sterilized seawater and/or marine Bushnell-Haas broth. Production of 14CO2 and CO2 was easily detectable during the first 48 hours with added hexadecane levels ranging from 10 to 500 mg/l of suspension and dependent on the biomass of hydrocarbon degraders, the hydrocarbon-oxidation potential of the biomass and nutrient availability. In addition to assessment of the hydrocarbon-degrading potential of environmental samples, the radiorespirometric procedure, and concomitant measurement of microbial biomass, has utility as an indicator of hydrocarbon contamination of soils, aqueous sediments and water, and can also be used to evaluate the effectiveness of bioremediation treatments. (Copyright (c) 1991 Kluwer Academic Publishers.)