Record Display for the EPA National Library Catalog

RECORD NUMBER: 11 OF 15

OLS Field Name OLS Field Data
Main Title Recovery of Bulk DNA from Soil by a Rapid, Small-Scale Extraction Method.
Author Porteous, L. A. ; Armstrong, J. L. ;
CORP Author Corvallis Environmental Research Lab., OR.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-91/225;
Stock Number PB92-108141
Additional Subjects Extraction ; Soils ; Deoxyribonucleic acids ; Separation ; Humic acids ; Centrifuging ; Ecology ; Reprints ; Lysozyme ; Novogym
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-108141 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 02/24/1992
Collation 6p
Abstract
The authors describe an extraction method that yields restrictable 20-25 kb DNA from one gram of soil. Cells are lysed directly in the soil. The crude DNA extract is separated from contaminating humic compounds, concentrated, and purified by CsCl gradient centrifugation and the commercial product Geneclean.