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RECORD NUMBER: 3 OF 3

OLS Field Name OLS Field Data
Main Title X-ray Microanalysis of Calcium, Potassium, and Phosphorus in Liver Mitochondria Stressed by Carbon Tetrachloride.
Author Millette, J. R. ; Allenspach, A. L. ; Clark, P. J. ; McCauley, P. T. ; Washington, I. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Miami Univ., Oxford, OH. Dept. of Zoology.
Year Published 1985
Report Number EPA/600/J-85/140;
Stock Number PB86-101177
Additional Subjects Carbon tetrachloride ; X ray analysis ; Microanalysis ; Calcium ; Potassium ; Phosphorus ; Toxicology ; Environmental surveys ; Bioassay ; In vivo analysis ; Laboratory animals ; Rats ; In vitro analysis ; Chemical analysis ; Liver ; Concentration(Composition) ; Reprints ; Environmental health ; Liver mitochondria
Holdings
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Status
NTIS  PB86-101177 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 8p
Abstract
Previous measurements of elemental concentrations in liver mitochondria have generally required homogenation and fractionation of liver tissue, a procedure in which it is difficult to rule out ion movement between subcellular units. New techniques involving cryo-ultramicrotomy of rapidly frozen tissue, high resolution scanning transmission electron microscopy and X-ray microanalysis were used to measure those elements in rat liver mitochondria reported to have changed following oral administration of carbon tetrachloride (CCl4). Increases in liver mitochondrial calcium were found 24 hours following intoxication by CCl4. Significant early (2 hours) mitochondrial increases in potassium and phosphorus were found following administration of CCl4. The electron microscope technique using quick frozen samples promises to allow measurement of intracellular ionic concentrations under virtually lifelike conditions.