| Main Title |
Comparison of Bioassay and Enzyme-Linked Immunosorbent Assay for Quantification of 'Spodoptera frugiperda' Nuclear Polyhedrosis Virus in Soil. |
| Author |
Kawanishi, C. ;
Fuxa, J. R. ;
Warren, G. W. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Louisiana State Univ., Baton Rouge. Dept. of Entomology. |
| Year Published |
1985 |
| Report Number |
EPA/600/J-85/396; |
| Stock Number |
PB86-202967 |
| Additional Subjects |
Bioassay ;
Viruses ;
Soils ;
Reprints ;
Immunosorbent assays ;
Spodoptera frugiperda
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB86-202967 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
7p |
| Abstract |
Standard curves with known amounts of Spodoptera frugiperda nuclear polyhedrosis virus (NPV) in soil were established with a bioassay and with an enzyme-linked immunosorbent assay (ELISA). The bioassay detected as few as 4 x 10 to the 4th power polyhedral inclusion bodies (PIB)/g of sandy soil and < 10 PIB/g of soils with large amounts of silt or clay. The ELISA detected as few as 360 PIB/g in all three soil types, and absorbance values were inversely related to the amount of clay. Results of the bioassay and ELISA were significantly (P < 0.01) correlated for natural NPV from field samples of silt (R equal 0.961) and sandy soil (R=0.723). Soil samples from Louisiana pastures and corn fields contain up to 7.6 times 10 to the 4th power PIB/g, and 2 times 10 to the 4th power PIB/g are commonly present. |
