Record Display for the EPA National Library Catalog


Main Title "In situ" characterization of microorganisms indegenous to water-tabe aquifers.
Author Balkwill, David. ; Ghiorse, W. C.
Other Authors
Author Title of a Work
Ghiorse, W. C.
CORP Author Florida State Univ., Tallahassee. Dept. of Biological Science. ;Cornell Univ., Ithaca, NY.;Robert S. Kerr Environmental Research Lab., Ada, OK.
Publisher NTIS,
Year Published 1984
Report Number EPA 600/D-84-232
Stock Number PB85-101731
OCLC Number 28756859
Additional Subjects Ground water ; Water pollution ; Soil microbiology ; Sampling ; Bacteria ; Counting methods ; Cells(Biology) ; Respiration ; Bioassay ; Reduction(Chemistry) ; Cell morphology ; Biological stains ; Water table ; Aquifers ; Electron microscopy ; Chlorine organic compounds ; Tetrazolium chloride/(iodophenyl)-(nitrophenyl)-phenyl
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
ELBD RPS EPA 600-D-84-232 repository copy AWBERC Library/Cincinnati,OH 10/17/2014
NTIS  PB85-101731 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13 p.
Core material from the deeper subsurface was examined for the presence and activity of microbes. Methods included acridine orange direct counts of the total number of cells, 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride reduction assays of the number of respiring cells, plate counts of the number of viable cells, and examination of the ultra-structural characteristics of any microbes by transmission electron microscopy. The results demonstrated conclusively that appreciable numbers (1-10 million per gram) of bacteria reside in shallow, water-table aquifers.
EPA 600/D-84-232. PB: 85-101731. Photocopy.