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Main Title Freshwater assay using soil eluates as sample material : (single laboratory evaluation) /
Author Lower, W. R. ; Thomas, M. W. ; Judy, B. M. ; Sutton, W. W.
Other Authors
Author Title of a Work
Lower, W. R.
CORP Author Environmental Research Lab., Athens, GA. ;Missouri Univ.-Columbia. Environmental Trace Substances Research Center.
Publisher U.S. Environmental Protection Agency, Environmental Research Laboratory,
Year Published 1990
Report Number EPA/600/3-90/038
Stock Number PB90-203456
Subjects Soils--United States--Testing ; Freshwater algae--United States--Analysis ; Hazardous wastes--United States--Measurement ; Hazardous waste sites--United States
Additional Subjects Hazardous materials ; Soils ; Bioassay ; Tests ; Chlorophyta ; Loam ; Clay soils ; Operations ; Monitoring ; Sodium fluorides ; Dosage ; Zinc chloride ; Response ; Measurement ; Acceptability
Library Call Number Additional Info Location Last
NTIS  PB90-203456 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
The Chlorophyta assay, which uses soil as sample material, has been a useful bioassessment technique for screening hazardous waste site problems. An eluate is prepared from a 125-gram soil sample and then diluted into three separate concentrations prior to being tested using Selenastrum capricornutum. The work reported here has attempted to determine the procedure's capability for data quality, to provide a basis for deciding whether the assay merits collaborative testing, and to define more clearly the method's potential for inclusion as part of an operational monitoring network. The soil used for most of the evaluation was a homogenized clay loam. Soil containing either sodium fluoride or 2,4-dichlorophenol was tested using the algal assay, and several tests were conducted to confirm the dose/response curve using the positive control compound, zinc chloride. A known test response also was established using a white silica sand spiked with sodium fluoride. Results revealed an apparently poor capability for sensitivity and a somewhat limited range of reliable measurement.
Caption title. "April 1990." "EPA/600/3-90/038." Microfiche.
Contents Notes