"Report date December 1971." Prepared for the United States Environmental Protection Agency. Includes bibliographical references (page 43).

In conjunction with already existing methods for measuring the chemical and physical characteristics of water, methods for rapidly measuring biological effects of water quality would be very useful. Thus the authors studied the effects of pollution on the breathing rates of bluegills. The presence of zinc at concentrations of 8.7, 5.22, 4.16 and 2.55 mg/l in dechlorinated municipal tapwater was detected by an increase in breathing rate or a change in breathing rate variance of bluegills. None of the fish exposed to the three lower concentrations died during the experiments. The criterion for detection was an arbitrary number of responses occurring at the same time. An in-plant system for the prevention of fish kills caused by spills could be developed by monitoring several biological functions of fish simultaneously.