Contents Notes |
The animal cap assay / Jeremy Green -- Cell and tissue transplantation in zebrafish embryos / Toshiro Mizuno, Minori Shinya, and Hiroyuki Takeda -- Ribonuclease protection analysis of gene expression in Xenopus / Craig S. Newman and Paul A. Krieg -- Quantitative analysis of mRNA levels in Xenopus embryos by reverse transcriptase-polymerase chain reaction (RT-PCR) / Oliver C. Steinbach and Ralph A.W. Rupp -- Wholemount in situ hybridization of Xenopus and zebrafish embryos / Joanne Broadbent and E. Mary Read -- In situ hybridization to sections of Xenopus embryos / David Bertwistle -- Zebrafish immunohistochemistry / Rachel Macdonald -- Immunohistochemistry of Xenopus embryos / Carl Robinson and Matt Guille -- Preparation and testing of synthetic mRNA for microinjection / Wendy Moore and Matt Guille -- Microinjection into Xenopus oocytes and embryos / Matt Guille -- Microinjection into zebrafish embryos / Qiling Xu -- Expression from DNA injected into Xenopus embryos / Ondine Cleaver and Paul A. Krieg -- Promoter analysis in zebrafish embryos / Jos Joore -- Transient transgenesis in Xenopus laevis facilitated by AAV-ITRs / Yuchang Fu, Donghui Kan, and Sylvia Evans -- Band-shift analysis using crude oocyte and embryo extracts from Xenopus laevis / Rob Orford and Matt Guille -- DNA-footprinting using crude embryonic extracts from Xenopus laevis / Rob Orford, Darren Mernagh, and Matt Guille -- Mapping protein-DNA interactions using in vivo footprinting / David Warshawsky and Leo Miller. |