Section 303(d) of the federal Clean Water Act (CWA) and the U.S. Environmental Protection Agency's (EPA) implementing regulations direct each state to identify and list waters, known as water quality limited segments (WQLSs), in which current required controls of a specified substance are inadequate to achieve water quality standards. This list of impaired waters is commonly referred to as the 303(d) list. For each WQLS, the State is to either establish a Total Maximum Daily Load (TMDL) for the specified substance that the waterbody can receive without violating water quality standards, or demonstrate that water quality standards are being met. Wills Creek (basin code 02141003), located in Allegany and Garrett Counties, Maryland, was identified on the States list of WQLSs as impaired by cyanide (CN) (1996 listing), sediments (1996 listing), nutrients (1996 listing), low pH (1998 listing), fecal coliform (2002 listing) and impacts to biological communities (2002 listing). The information used for listing CN is suspect due in part to sampling and analysis methods available at the time, and assessment inconsistencies that led to the listing in 1996. This report provides an analysis of recent monitoring data, which shows that the aquatic life criteria and designated uses associated with CN are being met in the Wills Creek watershed, and that the 303(d) impairment listings associated with CN are not supported by the analyses contained herein. A Water Quality Analysis (WQA) of CN for Wills Creek was conducted by MDE using recent water column chemistry data and sediment toxicity data to determine if an impairment currently exists.