Record Display for the EPA National Library CatalogRECORD NUMBER: 5 OF 12
|Main Title||Literature review of molecular methods for simultaneous detection of pathogens in water [electronic resource] /|
|CORP Author||Environmental Protection Agency, Washington, DC.|
|Publisher||National Risk Management Research Laboratory, Office of Research and Development U.S. Environmental Protection Agency,|
|Subjects||Water--Pollution--Measurement. ; Waterborne infection. ; Water-supply--Health aspects. ; Pathogenic microorganisms.|
|Additional Subjects||Water pollution monitoring ; Pathogens ; Water systems ; Drinking water ; Water supply ; Bacteria ; Disease outbreaks ; Death ; Microorganisms ; Contamination ; Infectious diseases ; Water pollution control ; Coliforms ; Turbidity|
|Collation|| p. : digital file, PDF|
Waterborne pathogens continue to contaminate drinking water supplies and cause waterborne disease outbreaks (WBDO) despite current regulations designed to prevent and control their spread. In the United States, from 1991-2000, 173 waterborne outbreaks and 432,733 cases of illness were reported in public and individual water systems. In 1993, an estimated 400,000 people became ill in Milwaukee, Wisconsin after drinking water contaminated with Cryptosporidium parvum, a waterborne pathogen that contaminates drinking water supplies. Annually, the CDC estimates that pathogen infected drinking water results in about a million new cases of illness and about a thousand deaths. Most cases, however, are not reported to health care providers because of their self-limiting nature and that do not result in WBDOs. EPA currently regulates two indicators of microbiological drinking water quality: total coliform and turbidity. A challenge for water utilities is that current indicators of water quality do not detect all types of microbial contamination since there is a great diversity of microbial pathogens.
"EPA/600/R-07/128" "February 2008." Title taken from title screen (viewed January 2, 2009).
[The report] is a review of molecular technologies (qPCR, microarray, microfluidics and lab-on-a-chip) for simultaneous detection of multiple waterborne pathogens in order to understand the state of the technology. The search content focuses on: Pathogen detection without DNA or RNA amplification, Pathogen detection with DNA or RNA amplification, Sample concentration methodologies, Whole methods (concentration and detection without DNA or RNA amplification), Whole methods (concentration and detection with DNA or RNA amplification), Molecular methods that differentiate viable versus non-viable microorganisms. While the primary focus is on waterborne pathogens in potable water, the literature search includes wastewater and emerging clinical methodologies as these may be transferable to potable water applications and reports on recent technological developments covering advances made in the last ten years.