Susquehanna River Basin Commission (SRBC) conducted a water quality and biological survey of the Upper Susquehanna Subbasin from June to September 2007. This survey is part of SRBCs Subbasin Survey Program, which is funded in part by the United States Environmental Protection Agency (USEPA). The Subbasin Survey Program consists of two year assessments in each of the six major subbasins on a rotating schedule. This report details the Year-1 survey, which consists of point-in-time water chemistry, macroinvertebrate, and habitat data collection and assessments of the major tributaries and areas of interest throughout the Upper Susquehanna Subbasin. The Year-2 survey will be conducted in the Tioughnioga River over a one-year time period beginning in summer 2008. The Year-2 survey is part of a larger monitoring effort associated with an environmental restoration effort at Whitney Point Lake. Previous SRBC surveys of the Upper Susquehanna Subbasin were conducted in 1998 (Stoe, 1999) and 1984 (McMorran, 1985).