The document is one of five training modules developed by the Office of Water Enforcement and Permits, U.S. Environmental Protection Agency (EPA) to introduce the National Pollutant Discharge Elimination System (NPDES) program to new inspectors. Information in each module provides training to an inspector unfamiliar with the NPDES program. The modules address the following topics: The Overview presents an overview of the entire NPDES program and briefly summarizes different types of inspections conducted under the program; Legal issues discusses the legal issues which must be addressed during an inspection and provides legal information to assist inspectors in performing their duties; Biomonitoring outlines the principles of biomonitoring and the role of biological testing in the inspection program; Sampling procedures details procedures to be used when conducting a sampling inspection; and Laboratory analysis outlines procedures and information necessary to perform an effective evaluation of a permittee's laboratory. The module is designed to introduce new inspectors to the concepts and practices of toxicity testing as it relates to CBIs (Compliance Biomonitoring Inspections). It is not intended to be a definitive source of material on toxicity testing; rather, its focus is on explaining the points pertinent to CBIs. After mastering the material in the module, an inspector should be able to define terms used in whole effluent toxicity testing, describe whole effluent toxicity testing procedures, list key aspects of toxicity critical to interpretation of results, and list guidance documents that can be used to implement CBIs. Appendix A contains questions and answers that an inspector can use to self-test him/herself after completing the module.

Shipping list no.: 90-622-P. "June 1990."