Main Title |
'Spodoptera frugiperda' Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases BamHI and HindIII. |
Author |
Loh, Lambert C. ;
Hamm, John J. ;
Huang, Eng-Shang ;
|
CORP Author |
North Carolina Univ. at Chapel Hill.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1981 |
Report Number |
EPA-R-806210; EPA-600/J-81-517; |
Stock Number |
PB82-153768 |
Additional Subjects |
Genes ;
Viruses ;
Maps ;
Microorganisms ;
Genetics ;
Reprints ;
Spodoptera frugiperda ;
Nuclear polyhedrosis viruses ;
Endonucleases
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB82-153768 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
The physical map for the genome of Spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases BamHI and HindIII. The ordering of the restriction fragments was accomplished by cross-blot hybridization of BamHI, HindIII, and EcoRI fragments. The alignment of the HindIII fragments within the BamHI map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. The results showed that the viral genome consisted of mainly unique sequences. In addition, the circular nature of the viral genome was reaffirmed. |