Record Display for the EPA National Library Catalog


Main Title Gauze Pad Method for the Isolation of Viruses From Inland and Estuarine Waters.
Author Mille, Louise T. ; Li, Oscar C. ;
CORP Author Rhode Island Univ., Kingston. Water Resources Center.
Year Published 1970
Report Number DI-14-01-001-1860 ;DI-14-31-001-3040; OWRR-A-025-RI; 00634,; A-025-RI(1)
Stock Number PB-195 310
Additional Subjects ( Viruses ; Sampling) ; ( Water pollution ; Viruses) ; Sampling ; Isolation ; Inland waterways ; Estuaries ; Rhode Island ; Elution ; Adsorption ; Flocculating ; Virus isolation methods
Library Call Number Additional Info Location Last
NTIS  PB-195 310 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 14p
The ultimate objective of this study was to examine the viral flora of waters from estuaries, inland ponds and streams of Rhode Island. However, prior to carrying out field examination, it was necessary to evaluate under controlled laboratory conditions these methods currently available for sampling, concentration and isolation of viruses with a view to selecting the most effective method or designing a method which would simplify sampling and improve recovery. The methods evaluated included the gauze pad technique, adsorption and elution from chemical flocs, combined flocculation and centrifugation, sonnication, and finally adsorption and elution from magnetic iron ore. The gauze pad was found to be inefficient for recovering virus from fresh water but its efficiency was enhanced slightly by the presence of inorganic salts in water, e.g., sea water. Combined flocculation and centrifugation improved viral recovery, but showed selectivity in the viruses recovered. The best results were obtained by adsorbing virus onto magnetic iron oxide followed by elution with blood serum solutions. (Author)