||Flow Cytometric Analysis of Mouse Spermatogenic Function Following Exposure to Ethylnitrosourea.
Evenson, D. P. ;
Higgins, P. J. ;
Gruenberg, D. ;
Ballachey, B. E. ;
||South Dakota State Univ., Brookings. ;Sloan-Kettering Research Inst., New York.;Health Effects Research Lab., Research Triangle Park, NC.
Flow cytometry ;
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The effects of the mutagenic agent ethylnitrosourea (ENU) on spermatogenic function and sperm chromatin structure were studied by flow cytometry and the results compared with sperm head morphology measurements. Groups of mice received daily exposures ranging from 0 to 75 mg/kg body weight x 5 days and were sacrificed 28 days later. Fresh testicular cell suspensions and epididymal sperm were stained with acridine orange (AO) and measured by flow cytometry. Sperm nuclei were isolated, fixed, rehydrated, and then either subjected to thermal stress or not prior to staining with AO. Body weights were unaffected by the chemical exposure while the testicular weights were reduced by about 50%. Two-parameter (DNA, RNA) flow cytometry measurements showed a dose-response relationship in the loss of certain cell types, particularly the elongated spermatids, from the testes of treated animals.