Record Display for the EPA National Library Catalog


Main Title In vivo and In vitro Intestinal Survival and Competition of Environmental 'Pseudomonas' Species.
Author George, S. E. ; Kohan, M. J. ; Walsh, D. B. ; Nelson, G. M. ; Whitehouse, D. A. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher Nov 88
Year Published 1988
Report Number EPA/600/D-88/257;
Stock Number PB89-129068
Additional Subjects Pseudomonas ; Genetic intervention ; Bacterial genetics ; Intestines ; Toxicity ; Laboratory animals ; Selection(Genetics) ; Mutations ; Metabolism ; Transfection ;
Library Call Number Additional Info Location Last
NTIS  PB89-129068 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
The main objective of the work is to evaluate various mutant and recombinant microorganisms and/or their metabolites and identify any adverse effects they could have on human health. One of the primary focuses is to develop new methods and to improve existing methods for monitoring exposure to engineered bacterial strains and/or genotoxic metabolites generated by these organisms. Several areas of research are being pursued. They include (1) competition and survival of mutant microorganisms, (2) the identification and effects of toxic metabolites produced by mutant or recombinant microorganism biodegradation products and the associated toxicity, (4) genetic transfer capabilities and mechanisms of interest to the microbial gut inhabitants, and (5) expression within intestinal flora of genes transferred from the mutant or recombinant microorganisms of interest. In general, the Pseudomonas spp. were poor competitors in the mouse intestinal tract. Even though their numbers decreased significantly over the test period, some of the strains were still recoverable 14 days after dosing. In vitro results are similar.