Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Implications for the Presence of Transforming Genes in Gonadal Tumors in Two Bivalve Mollusk Species.
Author Van Beneden, R. J. ; Gardner, G. R. ; Blake, N. J. ; Blair, D. G. ;
CORP Author Duke Univ. Medical Center, Durham, NC. Dept. of Cell Biology. ;Duke Univ., Beaufort, NC. Marine Lab. ;University of South Florida, St. Petersburg. ;National Cancer Inst., Frederick, MD. Frederick Cancer Research and Development Center.;Environmental Research Lab., Narragansett, RI.
Publisher c1993
Year Published 1993
Report Number EPA-R-816277-01-0; EPA/600/J-93/417;
Stock Number PB94-101581
Additional Subjects Genetic transformation ; Mollusca ; Seminoma ; Dysgerminoma ; Water pollution effects(Animals) ; Species diversity ; 3T3 cells ; Agricultural chemicals ; Transfection ; Oncogenes ; Neoplasm DNA ; Reprints ; Mya arenaria ; Mercenaria
Library Call Number Additional Info Location Last
NTIS  PB94-101581 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 02/27/1994
Collation 6p
Studies were initiated on oncogene activation in two bivalve species with high frequencies of histologically identifiable gonadal neoplasms. Pathological assessments identified epizootic seminomas and dysgerminomas in softshell clams (Mya arenaria) from three Maine estuarine sites contaminated by herbicides and in hardshell clams (Mercenaria) from the Indian River in Florida, an area of potential citrus agrochemical exposure. NIH3T3 transfection assays were used to examine DNA isolated from these molluscan tumors for the presence of activated oncogenes. DNAs isolated from advanced tumors in both species were able to transform NIH3T3 cells in a standard focus assay. These same cells were also able to form colonies in low concentrations of serum and induce tumors in athymic mice. Cells expanded from isolated foci demonstrated anchorage-independent growth in soft agar. The results of these studies indicate that DNA from the clam tumors is able to transform mouse fibroblasts, which suggests that a transforming gene is present in these tumor cells. Studies are under way to identify the gene(s) detected by these assays.