Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Genetic Basis for Using 'Tradescantia' Clone 4430 as an Environmental Monitor of Mutagens.
Author Emmerling-Thompson, M. ; Nawrocky, M. M. ;
CORP Author Brookhaven National Lab., Upton, NY.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA-600/J-80-334;
Stock Number PB82-127556
Additional Subjects Monitors ; Mutagens ; Genetics ; Assessments ; Hazards ; Plants(Botany) ; Reprints ; Mutagenesis ; Tradescantia Clone 4430
Library Call Number Additional Info Location Last
NTIS  PB82-127556 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 7p
The Tradescantia stamen hair system developed by the late Dr. Arnold H. Sparrow has been used in a wide variety of radiobiological studies, and more recently as an environmental monitor for assessing the potential genetic hazards of various gasesous chemicals of both industrial and natural origin. The use of this system as a genetic monitor necessitates a thorough genetic analysis of the marker employed to measure mutagenicity. The phenotypic change in color from blue to pink in either petal or stamen hair cells has been used as a genetic marker under the assumption that the petal and stamen hair cells are heterozygous for blue, and if the dominant allele for blue color mutates or is lost, the recessive allele determines that the daughter cells will be pink. It is the purpose of this communication to present the results of genetic test by conventional breeding methods involving the pink locus in Tradescantia clone 4430, the diploid clone used exclusively in the Mobile Monitoring Vehicle at Brookhaven National Laboratory. Confirmation of a genetic, as opposed to a physiological, origin of the pink color in the petal and stamen hair cells of Tradescantia clone 4430 is essential to evaluating the validity of this test system.