The need to develop, validate and utilize in vitro models to test chemicals for neurotoxic potential is widely appreciated. The lecture discusses the major advantages and disadvantages of using cell and tissue culture, the various in vitro models amenable for neurotoxicity studies, and the distinction between mechanistic and screening models. Major considerations for designing screening battery to evaluate neurotoxicants are discussed. Topics such as choice of appropriate cell models in the battery, choice and quantification of endpoints (i.e., cytotoxic and neurotoxic), and technical considerations in the design of the battery are discussed.