| Main Title |
Recovery of DNA from Soils and Sediments. |
| Author |
Steffan, R. J. ;
Goksoeyr, J. ;
Bej, A. K. ;
Atlas, R. M. ;
|
| CORP Author |
Louisville Univ., KY. Dept. of Biology. ;Bergen Univ. (Norway). Dept. of Microbiology and Plant Physiology.;Corvallis Environmental Research Lab., OR. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-93/292; |
| Stock Number |
PB93-222834 |
| Additional Subjects |
Deoxyribonucleic acids ;
Cells(Biology) ;
Recovery ;
Bacteria ;
Soil microbiology ;
Extraction ;
Purification ;
Test methods ;
Spectrophotometry ;
Absorption ;
Sediments ;
Thymidines ;
Labeled substances ;
Comparison ;
Humic acids ;
Isotopic labeling ;
Tables(Data) ;
Reprints ;
Lysis method ;
Organic soils
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-222834 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
Experiments were performed to evaluate the effectiveness of different methodological approaches for recovering DNA from soil and sediment bacterial communities; cell extraction followed by lysis and DNA recovery (cell extraction method) versus direct cell lysis and alkaline extraction to recover DNA (direct lysis method). Efficiency of DNA recovery by each method was determined by spectrophotometric absorbance using a tritiated thymidine tracer. With both procedures, the use of polyvinylpolypyrrolidone was important for the removal of humic compounds to improve the purity of the recovered DNA; without extensive purification, various restriction enzymes failed to cut added target DNA. Milligram quantities of high-purity DNA were recovered from 100-g samples of both soils and sediments by the direct lysis methods, which was a >1-order-of-magnitude-higher yield than by the cell extraction methods. (Copyright (c) 1988, American Society for Microbiology.) |
