Record Display for the EPA National Library Catalog
RECORD NUMBER: 13 OF 37Main Title | Initial-Phase Optimization for Bioremediation of Munition Compound-Contaminated Soils. | |||||||||||
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Author | Funk, S. B. ; Roberts, D. J. ; Crawford, D. L. ; Crawford, R. L. ; | |||||||||||
CORP Author | Idaho Univ., Moscow. Dept. of Bacteriology and Biochemistry.;Environmental Research Lab., Athens, GA. Office of Research and Development. | |||||||||||
Publisher | cJul 93 | |||||||||||
Year Published | 1993 | |||||||||||
Report Number | EPA-R-820804; EPA/600/J-94/474; | |||||||||||
Stock Number | PB95-137360 | |||||||||||
Additional Subjects | Soil pollution ; Explosives ; Degradation ; Remedial action ; Reprints ; TNT ; Triazines ; Cultures(Biology) ; Anaerobic processes ; pH ; Biotechnology ; Contamination ; Nitrogen organic compounds ; Nitro compounds ; Polymerization ; Tetraacocine | |||||||||||
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Collation | 9p | |||||||||||
Abstract | We examined the bioremediation of soils contaminated with the munition compounds 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraacocine by a procedure that produced anaerobic conditions in the soils and promoted biodegradation of nitroaromatic contaminants. This procedure consisted of flooding the soils with 50 mM phosphate buffer, adding starch as a supplemental carbon substrate, and incubating under static conditions. Aerobic heterotrophs, present naturally in the soil or added as an inoculum, quickly removed the oxygen from the static cultures, creating anaerobic conditions. Removal of parent TNT molecules from the soil cultures by the strictly anaerobic microflora occurred within 4 days. |