Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Non-Random Cell Killing in Cryopreservation: Implications for Performance of the Battery of Leukocyte Tests (BLT), 1. Toxic and Immunotoxic Effects.
Author Strauss., G. H. S. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-90/508;
Stock Number PB91-199943
Additional Subjects Cell survival ; Cryopreservation ; Longitudinal studies ; T lymphocytes ; Cell line ; Mutagens ; Toxins ; In vivo analysis ; In vitro analysis ; Reprints ; Battery of leukocyte tests
Library Call Number Additional Info Location Last
NTIS  PB91-199943 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 09/04/1991
Collation 17p
To eliminate between-tests error in longitudinal studies, for specimen sharing, convenient scheduling, etc., it is necessary to freeze freshly separated leukocytes as well as non-transformed, continuous T lymphocyte (CTL) lines. To test the efficacy of a programmable freezer (temperature falls at an optimal rate), freshly isolated lymphocytes and CTLs were each aliquoted into three sets of vials. The cells were thawed and/or washed and assayed for viability and T helper (T(sub h)0/T suppressor (T(sub s)) ratio. It is clear that omadequate freezing non-randomly damages cells of T cell subpopulations, T(sub h) being more sensitive than T(sub s). Further, it is proved that inadequate cryopreservation can confound results from a number of assessment methods owing to morphological and functional damages. The Battery of Leukocyte Tests (BLT) under development in the laboratory is designed to detect toxic, immunotoxic and genotoxic effects of in vivo mutagens exposure to human blood.