||Fabrication of Single Cell Protein from Cellulosic Wastes.
Daly, William H. ;
Ruiz, Jr., Leonard P. ;
||Louisiana State Univ., Baton Rouge. Dept. of Chemistry.;National Environmental Research Center, Cincinnati, Ohio.
Bacterial proteins ;
Nucleic acids ;
Food supplements ;
Synthetic food ;
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Techniques to produce a protein isolate from a cellulolytic bacteria were researched with the intent of producing a potential human food supplement. The use of chemical additives which cause the cells to flocculate enabling them to be removed from the fermentation media by a desludging centrifuge offers an inexpensive means of cell harvesting. Several flocculating agents which are effective at a concentration of 0.1% for flocculating Cellulomonas are reported. The addition of food grade proteins followed by a pH adjustment offers an alternative to chemical flocculation. In addition, acid and alkaline hydrolysis to remove nucleic acids from the bacterial protein was investigated.