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Main Title Quantitative Changes in the Synaptic Vesicle Proteins Synapsin I and p38 and the Astrocyte-Specific Protein Glial Fibrillary Acidic Protein are Associated with Chemical-Induced Injury to the Rat Central Nervous System (Journal Version).
Author Brock, T. O. ; O'Callaghan, J. P. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher 1987
Year Published 1987
Report Number EPA/600/J-87/394;
Stock Number PB89-106041
Additional Subjects Brain injuries ; Toxicity ; Neurochemistry ; Nerve tissue proteins ; Glial fibrillary acidic protein ; Astrocytes ; Synaptic vesicles ; Pathology ; Tin organic compounds ; Hippocampus ; Frontal cortex ; Reprints ; Synapsin I ; P38 protein
Library Call Number Additional Info Location Last
NTIS  PB89-106041 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Measurements of neuron-specific and glia-specific proteins were used to characterize chemical-induced injury to the rat CNS. Trimethyltin (TMT), a neurotoxicant which preferentially damages neurons in limbic structures, was employed to produce consistent, time-dependent, dose-related, cell-type-specific alterations in CNS morphology. Brain weights and histology were used to verify the cytopathological effects of TMT. Accompanying changes in two synaptic vesicle-associated proteins, synapsin I and p38, and the astrocyte-associated protein, glial fibrillary acidic protein (GFAP), were measured by radioimmunoassay (RIA). Immunohistochemistry of GFAP and incorporation of 3H-thymidine into GFAP-positive astrocytes also were used to characterize astrocytic responses to TMT-induced injury. Finally, quantitative two-dimensional PAGE was employed to detect additional proteins affected by TMT. Acute administration of TMT caused large dose- and time-dependent decreases in synapsin I and p38 in hippocampus; the same proteins were largely unaffected in a non-limbic structure, the frontal cortex.