| Main Title |
Sequence Similarities in the Genes Encoding Polychlorinated Biphenyl Degradation by 'Pseudomonas' Strain LB400 and 'Alcaligenes eutrophus' H850. |
| Author |
Yates, J. R. ;
Mondello, F. J. ;
|
| CORP Author |
General Electric Co., Schenectady, NY. Research and Development Center.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
c1989 |
| Year Published |
1989 |
| Report Number |
EPA/600/J-93/275; |
| Stock Number |
PB93-229201 |
| Additional Subjects |
Bacteria ;
Biodegradation ;
Polychlorinated biphenyls ;
Bacterial genes ;
DNA hybridization ;
Oxidation ;
Cell constitutents ;
Genetic engineering ;
Genetics ;
Microbiology ;
Biochemistry ;
Reprints ;
Pseudomonas strain LB400 ;
Alcaligenes eutrophus H850 ;
Congeners ;
Strains(Biology) ;
Gene sequences
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229201 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
5p |
| Abstract |
DNA-DNA hybridization was used to compare the Pseudomonas strain LB400 genes for polychlorinated biphenyl (PCB) degradation with those from seven other PCB-degrading strains. Significant hybridization was detected to the genome of Alcaligenes eutrophus H850, a strain similar to LB400 in PCB-degrading capability. These two organisms showed a strong conservation of restriction sites in the region of DNA encoding PCB metabolism. No other sequence similarities were detected in the two genomes. DNA from the other PCB-degrading strains showed no hybridization to the probe, which demonstrated the existence of at least two distinct classes of genes encoding PCB degradation. (Copyright (c) 1989, American Society for Microbiology.) |
