Record Display for the EPA National Library Catalog


Main Title Sequence Similarities in the Genes Encoding Polychlorinated Biphenyl Degradation by 'Pseudomonas' Strain LB400 and 'Alcaligenes eutrophus' H850.
Author Yates, J. R. ; Mondello, F. J. ;
CORP Author General Electric Co., Schenectady, NY. Research and Development Center.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-93/275;
Stock Number PB93-229201
Additional Subjects Bacteria ; Biodegradation ; Polychlorinated biphenyls ; Bacterial genes ; DNA hybridization ; Oxidation ; Cell constitutents ; Genetic engineering ; Genetics ; Microbiology ; Biochemistry ; Reprints ; Pseudomonas strain LB400 ; Alcaligenes eutrophus H850 ; Congeners ; Strains(Biology) ; Gene sequences
Library Call Number Additional Info Location Last
NTIS  PB93-229201 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 5p
DNA-DNA hybridization was used to compare the Pseudomonas strain LB400 genes for polychlorinated biphenyl (PCB) degradation with those from seven other PCB-degrading strains. Significant hybridization was detected to the genome of Alcaligenes eutrophus H850, a strain similar to LB400 in PCB-degrading capability. These two organisms showed a strong conservation of restriction sites in the region of DNA encoding PCB metabolism. No other sequence similarities were detected in the two genomes. DNA from the other PCB-degrading strains showed no hybridization to the probe, which demonstrated the existence of at least two distinct classes of genes encoding PCB degradation. (Copyright (c) 1989, American Society for Microbiology.)