Record Display for the EPA National Library Catalog

RECORD NUMBER: 17 OF 38

Main Title Method for Measuring Bacterial Resistance to Metals Employing Epifluorescent Microscopy.
Author Zelibor, J. L. ; Tamplin, M. ; Colwell, R. R. ;
CORP Author Maryland Univ. at Baltimore.;Environmental Research Lab., Gulf Breeze, FL.;Department of the Interior, Washington, DC.
Year Published 1987
Report Number DI-14-08-0001-G-884; EPA/600/J-87/363;
Stock Number PB88-219803
Additional Subjects Bacteria ; Metals ; Resistance ; Bioassay ; Tissue culture ; Reprints ; Metal sensitive strains
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NTIS  PB88-219803 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Abstract
A direct viable counting method has been developed which can be used to measure resistance of bacteria to metal (DVCMR bio-assay). Results obtained using DVCMR was compared with classical culture methods and proven superior. Evaluation of test strains resistant to arsenic or manganese showed a parabolic curve, whereas sensitive strains demonstrated a logarithmic curve fit. The DVCMR bio-assay method to determine percent resistance (%R) was approximately twice as sensitive for detecting metal-resistant strains and 23 times for metal-sensitive strains, compared with cultural methods. It is concluded that the DVCMR bio-assay may be better suited for use in microbial biogeochemistry, i.e., contamination monitoring and mineral prospecting, than existing cultural methods.