Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Metabolism of Benzo(a)Pyrene and Persistence of DNA Adducts in the Brown Bullhead 'Ictalurus nebulosus'.
Author Sikka, H. C. ; Steward, A. R. ; Kandaswami, C. ; Rutkowski, J. P. ; Zaleski, J. ;
CORP Author State Univ. of New York Coll. at Buffalo. Div. of Environmental Toxicology and Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.;Kentucky Univ., Lexington. Medical Center.;Rutgers - The State Univ., Piscataway, NJ. Dept. of Pharmacology and Toxicology.
Publisher c1991
Year Published 1991
Report Number EPA-R-813799; EPA/600/J-92/200;
Stock Number PB92-189067
Additional Subjects Benzo(a)pyrene ; Metabolism ; DNA adducts ; Toxicology ; Liver microsomes ; Thin layer chromatography ; Stereochemistry ; Glutathione ; Glucuronates ; Reprints ; Brown bullhead ; Ictalurus nebulosus ; Benzo(a)pyrene dihydrodiol
Library Call Number Additional Info Location Last
NTIS  PB92-189067 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/22/1992
Collation 6p
The in vitro metabolism of ((3)H)benzo(a)pyrene (BP) and ((14)C)benzo(a)pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. Compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the (-) enantiomer) and smaller amounts of BP-4,5-diol. BP phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. BP-7,8-diol was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. BP-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. The major adduct was anti-BPDE-deoxyguanosine. (Copyright (c) 1991 Pergamon Press.)