Four groups of 12 male and 12 female Sprague-Dawley rats were treated by whole body inhalation exposure for 6 hours at day, 5 days a week for 13 weeks with commercial hexane (hexane) at mean (S.D.) concentrations of 0, 897 (44.7), 2,997 (185.3) or 9,006 (386.0) ppm. The vapor generated was found to contain 15.5 to 16.1% methylcyclopentane and 51.5 to 53.3% n-hexane. The control group was treated similarly in all respects except it received air only. Approximately 1 to 2 hours after the first exposure, the animals were examined in a functional observational battery (FOB). This test was repeated prior to exposure on days 1, 7, 14, 35, 63 and 91. Motor activity testing was performed pre-study and on days 34, 62 and 90. Eight animals per sex per group were perfused for neuropathology evaluations, which were performed on six of the perfused animals in both the air control and 9,006 ppm hexane groups.