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Main Title Comparison of Bioassay and Enzyme-Linked Immunosorbent Assay for Quantification of 'Spodoptera frugiperda' Nuclear Polyhedrosis Virus in Soil.
Author Kawanishi, C. ; Fuxa, J. R. ; Warren, G. W. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Louisiana State Univ., Baton Rouge. Dept. of Entomology.
Year Published 1985
Report Number EPA/600/J-85/396;
Stock Number PB86-202967
Additional Subjects Bioassay ; Viruses ; Soils ; Reprints ; Immunosorbent assays ; Spodoptera frugiperda
Library Call Number Additional Info Location Last
NTIS  PB86-202967 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
Standard curves with known amounts of Spodoptera frugiperda nuclear polyhedrosis virus (NPV) in soil were established with a bioassay and with an enzyme-linked immunosorbent assay (ELISA). The bioassay detected as few as 4 x 10 to the 4th power polyhedral inclusion bodies (PIB)/g of sandy soil and < 10 PIB/g of soils with large amounts of silt or clay. The ELISA detected as few as 360 PIB/g in all three soil types, and absorbance values were inversely related to the amount of clay. Results of the bioassay and ELISA were significantly (P < 0.01) correlated for natural NPV from field samples of silt (R equal 0.961) and sandy soil (R=0.723). Soil samples from Louisiana pastures and corn fields contain up to 7.6 times 10 to the 4th power PIB/g, and 2 times 10 to the 4th power PIB/g are commonly present.