Record Display for the EPA National Library Catalog


Main Title Fertilization by Sperm Microinjection and Zona Drilling: Applications in the Basic and Clinical Sciences.
Author Perreault, S. D. ; Naish, S. J. ; Zirkin, B. R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;California Univ., Santa Barbara. Dept. of Biological Sciences. ;Johns Hopkins Univ., Baltimore, MD. Dept. of Population Dynamics.
Year Published 1988
Report Number EPA/600/D-88/096;
Stock Number PB88-211784
Additional Subjects Reproduction(Biology) ; Fertility ; Sperm microinjection ; Zona drilling method ; Sperm ; Eggs
Library Call Number Additional Info Location Last
NTIS  PB88-211784 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 25p
Experimental manipulation of fertilization provides an exciting research approach for studying mechanisms involved in sperm/egg interaction and holds great promise as a means for overcoming some forms of human infertility. The report will focus on three methods for assisted fertilization: (1) micro-injection of a spermatozoon (or sperm nucleus) directly into the ooplasm; (2) injection of motile sperm through the zona pellucida and into the perivitelline space (PVS); and (3) zona-drilling or the creation of a channel in the zona pellucida through which sperm may contact the oocyte membrane directly. The use of these methods to address a variety of questions in gamete and developmental biology, as well as to initiate embryonic development in animals and humans, will be reviewed.