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Main Title Human Bronchial Epithelial Cell Strain with Unusual In vitro Growth Potential Which Undergoes Neoplastic Transformation after SV40 T Antigen Gene Transfection.
Author Reddel, R. R. ; Hsu, I. C. ; Mass, M. J. ; Hukku, B. ; Gerwin, B. I. ;
CORP Author National Cancer Inst., Bethesda, MD. Div. of Cancer Etiology. ;Children's Medical Research Foundation, Camperdown (Australia). ;Medical Coll. of Ohio at Toledo. Dept. of Pathology. ;Maryland Univ. at Baltimore. School of Medicine.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1991
Year Published 1991
Report Number EPA-68-02-4456; EPA/600/J-92/255;
Stock Number PB92-206358
Additional Subjects Neoplastic cell transformation ; Bronchi ; Transfection ; Polyomavirus transforming antigens ; Epithelium ; In vitro analysis ; Immunofluorescence techniques ; Karyotyping ; Southern blotting ; Chromosome abnormalities ; Deoxyribonucleic acids ; Plasmids ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-206358 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
Bronchial epithelial cells were cultured from an individual with no evidence of malignant disease. These cells, designated HB56B, had a greatly extended in vitro life-span, being able to undergo 50 passages and 200 population doublings in contrast to the usual 3 to 4 passages and 20 to 30 population doublings characteristic of normal human bronchial epithelial cells. HB56B cells had karyotypic evidence of an amplified region on the short arm of chromosome II. Unlike normal bronchial epithelial cells, which undergo terminal squamous differentiation in vitro in response to fetal bovine serum, HB56B cells were only minimally affected by serum. These cells were readily established as an immortalized cell line, HB56B/5T, following transfection with a plasmid containing SV40 early region DNA. HB56B cells may offer an experimental system for the study of proliferation, differentiation, and senescence control in human bronchial epithelial cells.