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Main Title Analysis of X-ray-Induced HPRT Mutations in CHO Cells: Insertion and Deletions.
Author Fuscoe, J. C. ; Zimmerman, L. J. ; Fekete, A. ; Setzer, R. W. ; Rossiter, B. J. F. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;Semmelweis Univ. of Medicine, Budapest (Hungary). ;Baylor Coll. of Medicine, Houston, TX.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/437;
Stock Number PB93-141364
Additional Subjects Mutagenesis ; X-ray irradiation ; DNA damage ; Hypoxanthine phosphoribosyltransferase ; CHO cells ; DNA mutational analysis ; Mutations ; Base sequence ; Gene expression ; Polymerase chain reaction ; Southern blotting ; Exons ; Genetic recombination ; Chromosome deletion ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB93-141364 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 15p
Molecular alterations were examined in the hypoxanthine guanine phosphoribosyltransferase (hprt) gene of 41 independent X-ray-induced thioguanine-resistant (TGR) Chinese hamster ovary (CHO) cell clones. Rapid screening of the clones by multiplex polymerase chain reaction (PCR) for the presence or absence of exons revealed that the bases for the mutant phenotype were total gene deletion (26/41), partial gene deletion (4/41), and an insertion (1/41). Southern blot analysis confirmed the deletion data and revealed an additional class of mutants that had a gene disruption but retained all hprt exons (2/41). In addition, the DNA sequence of the insertion mutation was determined.